D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F397H

Expression Data

Yield (mg/mL): 0.6183

Kinetic Assay Data

**slope of F397H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09885	0.1065	0.1049
B	25.00	0.0633	0.07965	0.08865
C	8.33	0.0648	0.06405	0.0588
D	2.78	0.0408	0.0486	0.051
E	0.93	0.02085	0.03045	0.02835
F	0.31		0.0174	0.0183
G	0.10	0.00555	0.0075	0.00765
H	0.03	0.00045		0.0006

Yield (mg/mL): 0.6183 Dilution Factor: 10×

Protein purified on 12/13/2024 and assayed on 12/16/2024.
Data uploaded by AlyssaYanes and last updated on 2024-12-17 23:14:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1451	0.1468	0.1443
B	25.00	0.1451	0.1265	0.1272
C	8.33	0.1123	0.1155	0.117
D	2.78	0.1008	0.09	0.1008
E	0.93	0.04785	0.0432	0.045
F	0.31	0.0198	0.02085	0.0219
G	0.10	0.00825	0.00825	0.0087
H	0.03	0	0	0

Yield (mg/mL): 3.747 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 21:54:20.

Thermostability Assay Data

**slope of F397H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	0.04245	0.0438	0.04215
B	31.3	0.03555	0.0333	0.0303
C	33.6	0.02685	0.0279	0.02655
D	37.7	0.0114	0.0111	0.0114
E	42.4	0.00135	0.0012	0.00135
F	45.7		0.0006	0.0006
G	48.3	0		0.00045
H	50.0	0.0006	0.0006	0.0006

Protein purified on 12/13/2024 and assayed on 12/16/2024.
Data uploaded by AlyssaYanes and last updated on 2024-12-18 00:01:28.

Reference Data for WT

**slope of F397H A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	0.072	0.08295	0.0729
B	31.3	0.0852	0.0858	0.07845
C	33.6	0.07605	0.0759	0.08475
D	37.7	0.09195	0.0783	0.07785
E	42.4	0.0099	0.01005	0.0102
F	45.7	0.0006	0.00075	0.0006
G	48.3	0.0006	0.0006
H	50.0	0.00045		0.0006

Protein purified on and assayed on .
Data uploaded by mariannegs and last updated on 2024-12-17 22:26:16.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.