D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T218G

Expression Data

Yield (mg/mL): 1.778

Kinetic Assay Data

**slope of T218G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0725	0.0752	0.0725
B	25.00	0.0658	0.0526	0.0474
C	8.33	0.0351	0.0340	0.0353
D	2.78	0.0253	0.0340	0.0247
E	0.93		0.01740	0.01630
F	0.31	0.00530	0.00890	0.00770
G	0.10		0.003050	0.003150
H	0.03	0.001250	0.001250	0.001450

Yield (mg/mL): 1.778 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by lfarinsky and last updated on 2025-01-13 17:14:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2526	0.2612	0.2613
B	25.00	0.2033	0.2002	0.2105
C	8.33	0.1438	0.1569	0.1471
D	2.78	0.1040	0.0832	0.0942
E	0.93	0.04440	0.04145	0.04290
F	0.31	0.01710	0.01470	0.01980
G	0.10	0.007700	0.005300	0.007100
H	0.03	0.002600	0.002150	0.002300

Yield (mg/mL): 2.59 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by lfarinsky and last updated on 2025-01-13 17:13:04.

Design Data

Rosetta Score for T218G: 8.267

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.