D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I417H

Expression Data

Yield (mg/mL): 1.246

Kinetic Assay Data

**slope of I417H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0205	0.0129	0.0078
B	25.00		0.0163	0.0146
C	8.33	0.0135	0.0103	0.0144
D	2.78	0.0071	0.0078	0.0078
E	0.93	0.00275	0.00305	0.00335
F	0.31	0.00110	0.00120	0.00130
G	0.10	0.000500	0.000600	0.000550
H	0.03	0.000250		0.000250

Yield (mg/mL): 1.246 Dilution Factor: 10×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by lfarinsky and last updated on 2025-01-13 17:14:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2526	0.2612	0.2613
B	25.00	0.2033	0.2002	0.2105
C	8.33	0.1438	0.1569	0.1471
D	2.78	0.1040	0.0832	0.0942
E	0.93	0.04440	0.04145	0.04290
F	0.31	0.01710	0.01470	0.01980
G	0.10	0.007700	0.005300	0.007100
H	0.03	0.002600	0.002150	0.002300

Yield (mg/mL): 2.59 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by lfarinsky and last updated on 2025-01-13 17:13:04.

Design Data

Rosetta Score for I417H: 8.588

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.