D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R240A

Expression Data

Yield (mg/mL): 3.302

Kinetic Assay Data

**slope of R240A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3875	0.3745	0.3742
B	25.00	0.2588	0.2443	0.2608
C	8.33	0.1643	0.1657	0.1609
D	2.78	0.0798	0.0844	0.0821
E	0.93	0.03345	0.03245	0.03390
F	0.31	0.01430	0.01630	0.01520
G	0.10	0.006850	0.006300
H	0.03	0.001800	0.002450	0.002650

Yield (mg/mL): 3.302 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by amaduros and last updated on 2025-01-26 17:49:40.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2328	0.2381	0.2455
B	25.00
C	8.33	0.1775	0.1712	0.1504
D	2.78	0.1043	0.1030	0.1105
E	0.93	0.04690	0.03935	0.03970
F	0.31	0.02235	0.01880	0.01915
G	0.10	0.008750	0.006250	0.006550
H	0.03	0.002450	0.002550	0.002550

Yield (mg/mL): 1.614 Dilution Factor: 100×

Protein purified on 10/7/2024 and assayed on 10/9/2024.
Data uploaded by amaduros and last updated on 2025-01-15 23:12:33.

Design Data

Rosetta Score for R240A: 2.596

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.