D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V79T

Expression Data

Yield (mg/mL): 2.67

Kinetic Assay Data

**slope of V79T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.34E-02	2.81E-01	1.99E-01
B	25.00	1.49E-01	1.86E-01	1.25E-01
C	8.33	3.77E-02	1.07E-01	1.35E-01
D	2.78	8.32E-02	5.60E-02	7.35E-02
E	0.93	7.63E-02	5.30E-02	4.01E-02
F	0.31		2.18E-02	1.64E-02
G	0.10		7.09E-03	6.94E-03
H	0.03	1.10E-04		1.30E-04

Yield (mg/mL): 2.67 Dilution Factor: 100×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-16 11:10:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.32E-01	2.45E-01	2.14E-01
B	25.00	1.90E-01	1.69E-01	1.73E-01
C	8.33	1.52E-01	1.27E-01	1.56E-01
D	2.78	1.07E-01	1.17E-01	1.03E-01
E	0.93	8.74E-02	4.90E-02	7.09E-02
F	0.31	5.09E-02	2.46E-02	3.39E-02
G	0.10	1.34E-02	1.04E-02	1.49E-02
H	0.03	4.00E-05	1.10E-04	8.00E-05

Yield (mg/mL): 3.81 Dilution Factor: 100×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-15 23:07:21.

Design Data

Rosetta Score for V79T: 4.679

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.