D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T13K

Expression Data

Yield (mg/mL): 0.69

Kinetic Assay Data

**slope of T13K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.70E-04		8.70E-04
B	25.00	3.50E-04	7.20E-04	6.10E-04
C	8.33	5.10E-04	5.30E-04	7.00E-04
D	2.78	5.50E-04	5.20E-04	4.40E-04
E	0.93	3.60E-04	3.80E-04
F	0.31		2.00E-04	2.20E-04
G	0.10	1.40E-04	1.00E-04	0
H	0.03	1.30E-04	8.00E-05	1.70E-04

Yield (mg/mL): 0.69 Dilution Factor: 10×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-13 19:59:59.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.32E-01	2.45E-01	2.14E-01
B	25.00	1.90E-01	1.69E-01	1.73E-01
C	8.33	1.52E-01	1.27E-01	1.56E-01
D	2.78	1.07E-01	1.17E-01	1.03E-01
E	0.93	8.74E-02	4.90E-02	7.09E-02
F	0.31	5.09E-02	2.46E-02	3.39E-02
G	0.10	1.34E-02	1.04E-02	1.49E-02
H	0.03	4.00E-05	1.10E-04	8.00E-05

Yield (mg/mL): 3.81 Dilution Factor: 100×

Protein purified on 6/2/24 and assayed on 6/5/24.
Data uploaded by kuhumukherjee and last updated on 2025-01-15 23:07:21.

Design Data

Rosetta Score for T13K: 35.957

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.