D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A408K

Expression Data

Yield (mg/mL): 0.39

Kinetic Assay Data

**slope of A408K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.089550	0.0948	0.0852
B	25.00	0.0692	0.0746	0.0665
C	8.33	0.0579	0.0536	0.0583
D	2.78	0.0336	0.0334	0.0290
E	0.93	0.01155	0.01100	0.00980
F	0.31	0.00895	0.00720	0.00620
G	0.10	0.005350	0.005150	0.004900
H	0.03	0.006050	0.004200	0.005800

Yield (mg/mL): 0.39 Dilution Factor: 100×

Protein purified on 11/15/2024 and assayed on 11/18/2024.
Data uploaded by akalsbury and last updated on 2025-01-15 18:33:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.230500	0.2038	0.2119
B	25.00	0.1974	0.2072	0.1993
C	8.33	0.1727	0.1668	0.1647
D	2.78	0.1047	0.1038	0.0974
E	0.93		0.03560	0.03820
F	0.31	0.01225	0.01675	0.01380
G	0.10	0.006950	0.008150	0.007400
H	0.03	0.006200	0.005600	0.007550

Yield (mg/mL): 0.736 Dilution Factor: 100×

Protein purified on 11/15/2024 and assayed on 11/18/2024.
Data uploaded by akalsbury and last updated on 2025-01-22 22:25:11.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.