D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant G23A

Expression Data

Yield (mg/mL): 1.99

Kinetic Assay Data

**slope of G23A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.85E-01	1.74E-01	1.81E-01
B	25.00	1.45E-01	1.40E-01	1.31E-01
C	8.33	1.24E-01	1.09E-01	1.14E-01
D	2.78	1.28E-01		1.15E-01
E	0.93	7.43E-02		5.80E-02
F	0.31	3.92E-02		3.43E-02
G	0.10	1.92E-02	7.20E-03
H	0.03	3.00E-04	2.50E-04	3.50E-04

Yield (mg/mL): 1.99 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 15:36:30.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		8.79E-02	9.15E-02
B	25.00	8.64E-02	7.16E-02	7.52E-02
C	8.33	7.54E-02	7.23E-02	6.81E-02
D	2.78	5.46E-02	5.77E-02	4.57E-02
E	0.93	2.33E-02	3.20E-02	1.90E-02
F	0.31	7.25E-03		7.60E-03
G	0.10	5.85E-03	5.20E-03
H	0.03		1.50E-04	2.50E-04

Yield (mg/mL): 1.432 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 15:12:09.

Design Data

Rosetta Score for G23A: 1.771

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.