D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H59R

Expression Data

Yield (mg/mL): 1.53

Kinetic Assay Data

**slope of H59R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.36E-01	1.41E-01
B	25.00	1.38E-01	1.26E-01	1.23E-01
C	8.33	1.06E-01	9.10E-02	1.02E-01
D	2.78	7.04E-02	7.17E-02	7.20E-02
E	0.93	3.28E-02	3.20E-02	3.37E-02
F	0.31	1.28E-02	1.21E-02	1.25E-02
G	0.10	4.25E-03	4.30E-03	4.30E-03
H	0.03	3.00E-04	2.50E-04	2.50E-04

Yield (mg/mL): 1.53 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 16:11:48.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.23E-01	1.18E-01	1.14E-01
B	25.00	9.65E-02	1.00E-01	9.74E-02
C	8.33	8.97E-02	7.98E-02	9.02E-02
D	2.78	5.24E-02	4.70E-02	5.45E-02
E	0.93	2.74E-02	2.31E-02	2.55E-02
F	0.31	9.90E-03	8.65E-03	8.50E-03
G	0.10	3.75E-03	2.90E-03	3.10E-03
H	0.03		2.50E-04	2.50E-04

Yield (mg/mL): 1.446 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 15:55:02.

Design Data

Rosetta Score for H59R: -0.317

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.