D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S203V

Expression Data

Yield (mg/mL): 1.424

Kinetic Assay Data

**slope of S203V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	1.29E-01	1.13E-01	1.18E-01
C	8.33	9.48E-02	8.83E-02	9.17E-02
D	2.78	7.92E-02	8.46E-02	6.75E-02
E	0.93	3.36E-02	2.88E-02	2.88E-02
F	0.31	1.10E-02	1.21E-02	1.07E-02
G	0.10	4.70E-03	3.65E-03	3.85E-03
H	0.03		3.50E-04	4.00E-04

Yield (mg/mL): 1.424 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 16:37:40.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		8.12E-02	8.47E-02
C	8.33	7.32E-02	6.56E-02	6.36E-02
D	2.78		4.01E-02	3.71E-02
E	0.93	2.84E-02	2.01E-02	2.60E-02
F	0.31	1.21E-02	1.20E-02	8.75E-03
G	0.10	3.75E-03	3.70E-03	3.95E-03
H	0.03	0	0

Yield (mg/mL): 1.446 Dilution Factor: 100×

Protein purified on 1/30/2025 and assayed on 2/3/2025.
Data uploaded by AStrickland and last updated on 2025-02-05 16:28:40.

Design Data

Rosetta Score for S203V: -4.14

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.