D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N270G

Expression Data

Yield (mg/mL): 4.105

Kinetic Assay Data

**slope of N270G A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	2.83E-01	2.78E-01
B	25.00	2.31E-01	2.38E-01
C	8.33	1.81E-01	1.82E-01
D	2.78	1.49E-01	1.43E-01
E	0.93	5.19E-02	5.71E-02
F	0.31	2.00E-02	2.01E-02
G	0.10	6.75E-03	6.80E-03
H	0.03		2.00E-04

Yield (mg/mL): 4.105 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-20 14:44:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for N270G: 3.134

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.