D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant G271D

Expression Data

Yield (mg/mL): 3.835

Kinetic Assay Data

**slope of G271D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.24E-01	3.41E-01
B	25.00		2.57E-01	2.66E-01
C	8.33		2.06E-01	1.94E-01
D	2.78		1.71E-01	1.56E-01
E	0.93		5.77E-02	6.34E-02
F	0.31		2.12E-02	2.18E-02
G	0.10		7.40E-03	7.25E-03
H	0.03	0	0

Yield (mg/mL): 3.835 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-20 14:48:23.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for G271D: 4.871

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.