D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T303K

Expression Data

Yield (mg/mL): 4.755

Kinetic Assay Data

**slope of T303K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.63E-01	3.46E-01
B	25.00	3.92E-01	3.06E-01	3.35E-01
C	8.33	2.84E-01	2.54E-01	2.23E-01
D	2.78	2.36E-01	2.67E-01	1.90E-01
E	0.93	1.21E-01	1.22E-01	1.03E-01
F	0.31	5.37E-02	4.77E-02	3.97E-02
G	0.10	1.56E-02	1.43E-02	1.32E-02
H	0.03	3.50E-04	3.50E-04	2.50E-04

Yield (mg/mL): 4.755 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-20 15:07:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	2	3
A	75.00	3.08E-01	2.90E-01
B	25.00	2.77E-01	2.78E-01
C	8.33	2.52E-01	2.49E-01
D	2.78	1.55E-01	1.42E-01
E	0.93	7.52E-02	6.57E-02
F	0.31	2.61E-02	2.09E-02
G	0.10	9.20E-03	8.10E-03
H	0.03	2.00E-04	3.00E-04

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 2/13/2025 and assayed on 2/17/2025.
Data uploaded by AStrickland and last updated on 2025-02-19 15:42:15.

Design Data

Rosetta Score for T303K: 11.006

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.