D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q285A

Expression Data

Yield (mg/mL): 1.464

Kinetic Assay Data

**slope of Q285A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0044	0	0.0112
B	25.00	0.0036	0.0031	0.0030
C	8.33	0.0017	0.0017	0.0017
D	2.78	0.0007	0.0008	0.0008
E	0.93	0.00055	0.00055	0.00045
F	0.31	0.00030	0	0.00040
G	0.10	0.000250	0.000200	0.000250
H	0.03	0.000150	0.000200	0.000300

Yield (mg/mL): 1 Dilution Factor: 10×

Protein purified on 2/3/2025 and assayed on 2/4/2025.
Data uploaded by LoganPetit and last updated on 2025-02-24 16:38:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4148	0.4251	0.3884
B	25.00	0.3576	0.3671	0.3456
C	8.33	0.2606	0.2717	0.2890
D	2.78	0.1495	0.1926	0.2211
E	0.93	0.07400	0.08336	0.08720
F	0.31	0.03130	0.03215	0.03395
G	0.10	0.010600	0.012100	0.014100
H	0.03	0.000900	0.000800

Yield (mg/mL): 4.6 Dilution Factor: 100×

Protein purified on 2/3/2025 and assayed on 2/4/2025.
Data uploaded by LoganPetit and last updated on 2025-02-24 15:54:07.

Design Data

Rosetta Score for Q285A: 7.125

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.