D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D279Y

Expression Data

Yield (mg/mL): 0.072

Kinetic Assay Data

**slope of D279Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.00E-04	5.00E-04	5.00E-04
B	25.00	1.48E-16		1.48E-16
C	8.33	0.00E+00		7.40E-17
D	2.78	0.00E+00		0.00E+00
E	0.93	0.00E+00	5.00E-04
F	0.31	5.00E-04		5.00E-04
G	0.10	5.00E-04	5.00E-04	5.00E-04
H	0.03	0.00E+00		0.00E+00

Yield (mg/mL): 0.072 Dilution Factor: 10×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by BGrause and last updated on 2025-04-10 13:46:16.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.84E-01	2.93E-01	2.37E-01
B	25.00	1.82E-01	2.18E-01	1.88E-01
C	8.33	1.45E-01	1.56E-01	1.75E-01
D	2.78	1.32E-01	8.15E-02	6.35E-02
E	0.93	3.40E-02	3.40E-02	2.90E-02
F	0.31	1.30E-02	1.25E-02	1.40E-02
G	0.10	4.50E-03	4.00E-03	3.00E-03
H	0.03		1.48E-16	0.00E+00

Yield (mg/mL): 1.565 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by DavidHoyt and last updated on 2025-04-10 11:46:29.

Design Data

Rosetta Score for D279Y: 5.207

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.