D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K413H

Expression Data

Yield (mg/mL): 1.3

Kinetic Assay Data

**slope of K413H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0436	0.0520	0.0593
B	25.00	0.0454	0.0422	0.0446
C	8.33	0.0283	0.0186	0.0233
D	2.78	0.0104	0.0111	0.0107
E	0.93	0.00440	0.00460	0.00370
F	0.31	0.00135	0.00150	0.00125
G	0.10	0.000700	0.000800	0.000650
H	0.03	0.000200		0.000350

Yield (mg/mL): 1.3 Dilution Factor: 100×

Protein purified on 2/24/2025 and assayed on 2/28/2025.
Data uploaded by lfarinsky and last updated on 2025-03-06 17:33:44.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2547	0.2471	0.2920
B	25.00	0.2188	0.2281	0.2135
C	8.33	0.1790	0.1543	0.1495
D	2.78	0.0826	0.0842	0.0903
E	0.93	0.03765	0.03415	0.03825
F	0.31	0.01345	0.01090	0.01350
G	0.10	0.003850	0.003550	0.004550
H	0.03	0	0.000150	0.000450

Yield (mg/mL): 3.382 Dilution Factor: 100×

Protein purified on 2/24/2025 and assayed on 2/28/2025.
Data uploaded by lfarinsky and last updated on 2025-03-06 17:27:55.

Design Data

Rosetta Score for K413H: 7.204

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.