D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y173L

Expression Data

Yield (mg/mL): 0.526

Kinetic Assay Data

**slope of Y173L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.25E-01	1.36E-01	1.23E-01
B	25.00	1.07E-01	1.09E-01	1.05E-01
C	8.33	7.80E-02	8.10E-02	8.40E-02
D	2.78	4.55E-02	4.85E-02	4.60E-02
E	0.93	2.10E-02	2.80E-02	2.00E-02
F	0.31	8.00E-03	8.50E-03	8.50E-03
G	0.10	3.00E-03	3.50E-03	3.00E-03
H	0.03	1.48E-16	1.50E-03	5.00E-04

Yield (mg/mL): 0.526 Dilution Factor: 10×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by emgraham and last updated on 2025-04-10 11:57:49.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	9.27E-01
C	8.33	8.98E-01	9.49E-01	8.56E-01
D	2.78	4.52E-01	5.18E-01	5.16E-01
E	0.93	2.35E-01	2.22E-01	2.08E-01
F	0.31	6.85E-02	7.30E-02	7.90E-02
G	0.10	3.40E-02	2.55E-02	2.90E-02
H	0.03	5.00E-04		2.00E-03

Yield (mg/mL): 10.867 Dilution Factor: 100×

Protein purified on 3/27/2025 and assayed on 4/3/2025.
Data uploaded by emgraham and last updated on 2025-04-10 11:47:04.

Design Data

Rosetta Score for Y173L: 10.101

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.