Raw Data for BglB Variant W399H
Expression Data
0.187
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.95E-02 | 1.90E-02 | 1.80E-02 | ||
| B | 25.00 | 1.25E-02 | 1.20E-02 | 1.25E-02 | ||
| C | 8.33 | 7.00E-03 | 7.00E-03 | 7.00E-03 | ||
| D | 2.78 | 4.00E-03 | 4.50E-03 | 4.00E-03 | ||
| E | 0.93 | 2.00E-03 | 2.00E-03 | 2.00E-03 | ||
| F | 0.31 | 1.00E-03 | 5.00E-04 | 1.50E-03 | ||
| G | 0.10 | 5.00E-04 | 5.00E-04 | |||
| H | 0.03 | 7.40E-17 | 0.00E+00 |
Protein purified on 2025-02-05 and assayed on 2025-03-25.
Data uploaded by lucasclee and last updated on 2025-04-15 13:41:44.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 8.70E-02 | 8.25E-02 | 8.00E-02 | ||
| B | 25.00 | 6.85E-02 | 6.70E-02 | 6.50E-02 | ||
| C | 8.33 | 5.00E-02 | 4.90E-02 | 4.80E-02 | ||
| D | 2.78 | 2.90E-02 | 3.05E-02 | 3.65E-02 | ||
| E | 0.93 | 1.30E-02 | 1.30E-02 | 1.25E-02 | ||
| F | 0.31 | 5.00E-03 | 5.00E-03 | 5.00E-03 | ||
| G | 0.10 | 2.00E-03 | 2.00E-03 | 2.00E-03 | ||
| H | 0.03 | 1.00E-03 | 5.00E-04 | 7.40E-17 |
Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by IsabelleStaco and last updated on 2025-04-15 14:22:18.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | |||||
| B | 48.4 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| C | 45.9 | 1.00E-03 | 1.00E-03 | |||
| D | 42.2 | 1.00E-03 | 1.00E-03 | 1.00E-03 | ||
| E | 37.4 | 1.50E-03 | 1.50E-03 | |||
| F | 33.8 | 1.50E-03 | 1.50E-03 | |||
| G | 31.3 | 2.00E-03 | 2.00E-03 | 2.00E-03 | ||
| H | 30.0 | 1.50E-03 | 1.50E-03 | 1.50E-03 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 5.00E-03 | 6.00E-03 | 6.00E-03 | ||
| B | 48.4 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| C | 45.9 | 1.00E-03 | 1.50E-03 | 2.50E-03 | ||
| D | 42.2 | 1.05E-02 | 1.45E-02 | 1.25E-02 | ||
| E | 37.4 | 2.50E-02 | 2.05E-02 | 2.30E-02 | ||
| F | 33.8 | 2.80E-02 | 2.45E-02 | 2.50E-02 | ||
| G | 31.3 | 2.90E-02 | 3.00E-02 | 2.95E-02 | ||
| H | 30.0 | 2.50E-02 | 2.10E-02 | 2.45E-02 |
Protein purified on 2/25/25 and assayed on 4/1/25.
Data uploaded by IsabelleStaco and last updated on 2025-04-15 14:16:49.
Design Data
Citations
The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.