Raw Data for BglB Variant T212N

Expression Data

Yield (mg/mL): 0.2067

Thermostability Assay Data

slope of T212N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-04	5.00E-04	5.00E-04
	B	48.3	7.40E-17		0.00E+00
	C	45.7	1.50E-03	1.50E-03	1.50E-03
	D	42.4	4.55E-02	5.65E-02	5.60E-02
	E	37.7	1.78E-01	1.84E-01	1.83E-01
	F	33.6	1.84E-01	1.75E-01	1.67E-01
	G	31.6	1.96E-01		1.93E-01
	H	30.0	1.84E-01	1.96E-01	2.00E-01

Download these data

Protein purified on 25/02/2025 and assayed on 01/04/2025.
Data uploaded by debbiemo21 and last updated on 2025-04-15 14:46:15.

Reference Data for WT

slope of T212N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-04	5.00E-04	5.00E-04
	B	48.3	5.00E-04		5.00E-04
	C	45.7	4.50E-03	4.00E-03	3.50E-03
	D	42.4	1.62E-01	1.57E-01	1.75E-01
	E	37.7	3.41E-01	3.54E-01	3.40E-01
	F	33.6	3.83E-01	3.85E-01	3.86E-01
	G	31.3	3.55E-01	3.45E-01	3.56E-01
	H	30.0	3.72E-01	3.75E-01	3.70E-01

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by angelinaguirgis and last updated on 2025-04-15 14:34:43.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.