Raw Data for BglB Variant T116A

Expression Data

SDS-PAGE
 0.751

Kinetic Assay Data

slope of T116A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.95E-02 1.50E-02
B 25.00 7.00E-03 1.00E-02
C 8.33 5.00E-03 5.00E-03 5.00E-03
D 2.78 3.00E-03 3.00E-03
E 0.93 1.50E-03 1.50E-03
F 0.31 1.00E-03 1.00E-03
G 0.10 5.00E-04 5.00E-04
H 0.03
 0.552      100×

Protein purified on 3/1/25 and assayed on 3/28/25.
Data uploaded by nadiadrutau and last updated on 2025-04-17 08:08:47.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of T116A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.50E-03 2.00E-03 2.50E-03
B 48.3 5.00E-04 5.00E-04
C 45.7 0 1.48E-16
D 42.4 2.00E-03 2.00E-03
E 37.7 6.20E-02 6.20E-02 6.65E-02
F 33.6 1.26E-01 1.23E-01 1.19E-01
G 31.3 1.27E-01 1.29E-01 1.31E-01
H 30.0 1.24E-01 1.20E-01 1.23E-01

Protein purified on 2/27/25 and assayed on 4/7/25.
Data uploaded by nadiadrutau and last updated on 2025-04-17 08:23:55.


Reference Data for WT

slope of T116A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.50E-03 3.00E-03 3.50E-03
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 5.00E-04 5.00E-04
D 42.4 1.65E-02 1.80E-02 1.55E-02
E 37.7 3.25E-02 3.25E-02 3.25E-02
F 33.6 4.20E-02 4.10E-02 3.75E-02
G 31.3 4.05E-02 4.00E-02 3.60E-02
H 30.0 3.70E-02 2.90E-02 3.35E-02

Protein purified on 2/27/25 and assayed on 4/7/25.
Data uploaded by nadiadrutau and last updated on 2025-04-17 08:32:17.


Design Data

5.932

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.