Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.123

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.70E-02	8.25E-02	8.00E-02
	B	25.00	6.85E-02	6.70E-02	6.50E-02
	C	8.33	5.00E-02	4.90E-02	4.80E-02
	D	2.78	2.90E-02	3.05E-02	3.65E-02
	E	0.93	1.30E-02	1.30E-02	1.25E-02
	F	0.31	5.00E-03	5.00E-03	5.00E-03
	G	0.10	2.00E-03	2.00E-03	2.00E-03
	H	0.03	1.00E-03	5.00E-04	7.40E-17

Yield (mg/mL): 0.0921     Dilution Factor:  10×

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Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by IsabelleStaco and last updated on 2025-04-15 14:22:18.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-03	6.00E-03	6.00E-03
	B	48.4	5.00E-04	5.00E-04	5.00E-04
	C	45.9	1.00E-03	1.50E-03	2.50E-03
	D	42.2	1.05E-02	1.45E-02	1.25E-02
	E	37.4	2.50E-02	2.05E-02	2.30E-02
	F	33.8	2.80E-02	2.45E-02	2.50E-02
	G	31.3	2.90E-02	3.00E-02	2.95E-02
	H	30.0	2.50E-02	2.10E-02	2.45E-02

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Protein purified on 2/25/25 and assayed on 4/1/25.
Data uploaded by IsabelleStaco and last updated on 2025-04-15 14:16:49.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.