Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.094

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0305	0.0315	0.0315
	B	25.00	0.028	0.031	0.025
	C	8.33	0.017	0.017	0.017
	D	2.78	0.01	0.01	0.0105
	E	0.93	0.0045	0.0045	0.004
	F	0.31	0.002	0.002	0.002
	G	0.10	0.0005	0.0005
	H	0.03		7.401E-17	7.401E-17

Yield (mg/mL): 0.094     Dilution Factor:  100×

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Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by scobioma and last updated on 2025-04-15 17:04:37.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3		0.0005	0.0005
	C	45.7	0.0005	0.0005
	D	42.4	0.0155	0.0145	0.0135
	E	37.7	0.037	0.033	0.034
	F	33.6	0.046	0.0455	0.0415
	G	31.3	0.0515	0.053	0.042
	H	30.0	0.0525	0.0505	0.042

Download these data

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by scobioma and last updated on 2025-04-15 17:14:25.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.