Raw Data for BglB Variant R414D

Expression Data

Yield (mg/mL): 0.0951

Kinetic Assay Data

slope of R414D A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.85E-02	1.75E-02	1.75E-02
	B	25.00	1.05E-02	1.05E-02	1.00E-02
	C	8.33	5.50E-03	5.00E-03	5.00E-03
	D	2.78	2.50E-03	2.50E-03	2.50E-03
	E	0.93	1.00E-03	1.00E-03
	F	0.31	5.00E-04	5.00E-04
	G	0.10	0.00E+00		0.00E+00
	H	0.03

Yield (mg/mL): 0.06993     Dilution Factor:  10×

Download these data

Protein purified on 2/27/2025 and assayed on 3/27/2025.
Data uploaded by KaylaYale and last updated on 2025-04-17 13:07:17.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.