D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E154F

Yield (mg/mL): 0.263

**slope of E154F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0415	0.0435	0.0465
B	25.00	0.0375	0.0305	0.0345
C	8.33	0.026	0.023	0.022
D	2.78	0.0125	0.013	0.0125
E	0.93	0.006	0.006	0.006
F	0.31	0.003	0.0025	0.0025
G	0.10		0.001	0.001
H	0.03	0	0	0

Yield (mg/mL): 0.193 Dilution Factor: 100×

Protein purified on 3/6/25 and assayed on 3/27/25.
Data uploaded by hpatel and last updated on 2025-04-17 13:21:33.

**slope of E154F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0005	0.0005
B	48.3	0.0005	0.0005	0.0005
C	45.7	0.0005		0.0005
D	42.4	0.0025	0.002	0.002
E	37.7	0.016	0.0125	0.015
F	33.6	0.0205	0.021	0.02
G	31.3	0.0185	0.021	0.0205
H	30.0	0.0205	0.021	0.021

Protein purified on 3/6/25 and assayed on 4/3/25.
Data uploaded by hpatel and last updated on 2025-04-17 13:27:46.

**slope of E154F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3		0.0005	0.0005
C	45.7	0.0005	0.0005
D	42.4	0.0155	0.0145	0.0135
E	37.7	0.037	0.033	0.034
F	33.6	0.046	0.0455	0.0415
G	31.3	0.0515	0.053	0.042
H	30.0	0.0525	0.0505	0.042

Protein purified on 3/6/25 and assayed on 4/3/25.
Data uploaded by hpatel and last updated on 2025-04-17 13:38:23.

Rosetta Score for E154F: 3.077

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.