Raw Data for BglB Variant A226E

Expression Data

SDS-PAGE
 0.151

Kinetic Assay Data

slope of A226E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.30E-02 4.10E-02 4.15E-02
B 25.00 3.15E-02 3.10E-02 3.10E-02
C 8.33 2.40E-02 2.40E-02
D 2.78 1.50E-02 1.40E-02 1.45E-02
E 0.93 6.00E-03 6.50E-03 6.50E-03
F 0.31 3.00E-03 3.00E-03 3.00E-03
G 0.10 1.00E-03 1.00E-03
H 0.03 0.00E+00 0.00E+00 0.00E+00
 0.111      100×

Protein purified on 4/2/25 and assayed on 4/2/25.
Data uploaded by nusaibaislam and last updated on 2025-04-17 14:33:50.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.50E-02 4.60E-02 4.65E-02
B 25.00 3.75E-02 3.80E-02 3.80E-02
C 8.33 2.85E-02 2.70E-02 2.65E-02
D 2.78 1.80E-02 1.60E-02 1.65E-02
E 0.93 8.50E-03 7.00E-03 7.50E-03
F 0.31 3.50E-03 3.50E-03 3.50E-03
G 0.10 2.00E-03 1.50E-03 1.00E-03
H 0.03 0.00E+00 0.00E+00
 0.1338      100×

Protein purified on 4/2/25 and assayed on 4/2/25.
Data uploaded by nusaibaislam and last updated on 2025-04-17 14:29:45.


Thermostability Assay Data

slope of A226E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.48E-16 1.48E-16
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 0.00E+00 0.00E+00 0.00E+00
D 42.4 2.00E-03 1.00E-03 1.50E-03
E 37.7 1.40E-02 1.35E-02 1.30E-02
F 33.6 2.70E-02 2.75E-02 2.60E-02
G 31.3 3.15E-02 3.00E-02 2.95E-02
H 30.0 3.10E-02 3.15E-02 3.05E-02

Protein purified on 4/3/25 and assayed on 4/3/25.
Data uploaded by nusaibaislam and last updated on 2025-04-17 14:39:04.


Reference Data for WT

slope of A226E A420 nm (min−1)
T (°C) 1 2 3
A 30.0 1.40E-02 1.45E-02 1.60E-02
B 31.3 1.35E-02 1.45E-02 1.40E-02
C 33.6 1.40E-02 1.50E-02 1.50E-02
D 37.7 1.30E-02 1.30E-02 1.20E-02
E 42.4 0.0045 0.0045
F 45.7 0.0005 0.0005 0.0005
G 48.3 0 0.0005 0.001
H 50.0 1.48e-16 1.48e-16

Protein purified on 2/27/2025 and assayed on 4/3/2025.
Data uploaded by KaylaYale and last updated on 2025-04-17 13:27:05.


Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.