Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.181

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.50E-02	4.60E-02	4.65E-02
	B	25.00	3.75E-02	3.80E-02	3.80E-02
	C	8.33	2.85E-02	2.70E-02	2.65E-02
	D	2.78	1.80E-02	1.60E-02	1.65E-02
	E	0.93	8.50E-03	7.00E-03	7.50E-03
	F	0.31	3.50E-03	3.50E-03	3.50E-03
	G	0.10	2.00E-03	1.50E-03	1.00E-03
	H	0.03		0.00E+00	0.00E+00

Yield (mg/mL): 0.1338     Dilution Factor:  100×

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Protein purified on 4/2/25 and assayed on 4/2/25.
Data uploaded by nusaibaislam and last updated on 2025-04-17 14:29:45.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		1.48E-16	1.48E-16
	B	48.3	0.00E+00	5.00E-04	1.00E-03
	C	45.7	5.00E-04	5.00E-04	5.00E-04
	D	42.4	4.50E-03	4.50E-03
	E	37.7	1.30E-02	1.30E-02	1.20E-02
	F	33.6	1.40E-02	1.50E-02	1.50E-02
	G	31.3	1.35E-02	1.45E-02	1.40E-02
	H	30.0	1.40E-02	1.45E-02	1.60E-02

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Protein purified on 4/3/25 and assayed on 4/3/25.
Data uploaded by nusaibaislam and last updated on 2025-04-17 14:32:35.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.