Raw Data for BglB Variant T212N
Expression Data
0.11115
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.80E-02 | 2.75E-02 | 2.70E-02 | ||
| B | 25.00 | 2.05E-02 | 2.30E-02 | 2.50E-02 | ||
| C | 8.33 | 1.35E-02 | 1.55E-02 | 1.65E-02 | ||
| D | 2.78 | 7.50E-03 | 9.50E-03 | 9.00E-03 | ||
| E | 0.93 | 3.00E-03 | 4.00E-03 | 4.00E-03 | ||
| F | 0.31 | 1.50E-03 | 2.00E-03 | 2.00E-03 | ||
| G | 0.10 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| H | 0.03 | 0.00E+00 | 0.00E+00 |
Protein purified on 2/25/2025 and assayed on 3/25/2025.
Data uploaded by SethThomas and last updated on 2025-04-17 15:07:42.
Thermostability Assay Data
Data missing or deleted from the database.
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| B | 48.3 | 5.00E-04 | 5.00E-04 | |||
| C | 45.7 | 4.50E-03 | 4.00E-03 | 3.50E-03 | ||
| D | 42.4 | 1.62E-01 | 1.57E-01 | 1.75E-01 | ||
| E | 37.7 | 3.41E-01 | 3.54E-01 | 3.40E-01 | ||
| F | 33.6 | 3.83E-01 | 3.85E-01 | 3.86E-01 | ||
| G | 31.3 | 3.55E-01 | 3.45E-01 | 3.56E-01 | ||
| H | 30.0 | 3.72E-01 | 3.75E-01 | 3.70E-01 |
Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by angelinaguirgis and last updated on 2025-04-15 14:34:43.
Design Data
Citations
The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.