D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220K

Expression Data

Yield (mg/mL): 3.556

Kinetic Assay Data

**slope of N220K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.33E-01	4.11E-01	3.83E-01
B	25.00	3.08E-01	2.89E-01	3.08E-01
C	8.33	2.63E-01	2.85E-01	2.66E-01
D	2.78	2.34E-01	1.75E-01	2.02E-01
E	0.93	1.55E-01	1.16E-01	9.47E-02
F	0.31	8.05E-02	8.29E-02
G	0.10	4.10E-02	4.27E-02
H	0.03	0	0

Yield (mg/mL): 3.556 Dilution Factor: 100×

Protein purified on 11/2/21 and assayed on 11/4/21.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.49E-01	3.96E-01	3.77E-01
B	25.00	3.86E-01	3.51E-01	2.92E-01
C	8.33	2.68E-01	2.72E-01	2.77E-01
D	2.78	1.31E-01	1.43E-01	1.81E-01
E	0.93	6.97E-02	7.32E-02	7.95E-02
F	0.31	3.93E-02	3.31E-02	3.97E-02
G	0.10	1.19E-02	1.10E-02	1.23E-02
H	0.03	0	0

Yield (mg/mL): 3.71 Dilution Factor: 100×

Protein purified on 11/2/21 and assayed on 11/4/21.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.