D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H119M

Expression Data

Yield (mg/mL): 0.548

Kinetic Assay Data

**slope of H119M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.44E-02	1.40E-02	1.31E-02
B	25.00	8.70E-03	6.50E-03	7.55E-03
C	8.33	5.70E-03	5.40E-03	5.55E-03
D	2.78	4.20E-03	3.55E-03	4.55E-03
E	0.93	1.30E-03		1.15E-03
F	0.31	6.00E-04		6.50E-04
G	0.10	4.50E-04		4.50E-04
H	0.03	0	0

Yield (mg/mL): 0.548 Dilution Factor: 10×

Protein purified on 11/2/21 and assayed on 11/4/21.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.49E-01	3.96E-01	3.77E-01
B	25.00	3.86E-01	3.51E-01	2.92E-01
C	8.33	2.68E-01	2.72E-01	2.77E-01
D	2.78	1.31E-01	1.43E-01	1.81E-01
E	0.93	6.97E-02	7.32E-02	7.95E-02
F	0.31	3.93E-02	3.31E-02	3.97E-02
G	0.10	1.19E-02	1.10E-02	1.23E-02
H	0.03	0	0

Yield (mg/mL): 3.71 Dilution Factor: 100×

Protein purified on 11/2/21 and assayed on 11/4/21.
Data uploaded by cnvrodriguez and last updated on 2022-06-27 07:04:03.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.