Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3639	0.3318	0.3155
	B	25.00	0.2994	0.2659	0.2694
	C	8.33		0.1747	0.177
	D	2.78	0.0708	0.0672	0.06525
	E	0.93	0.0216	0.0204	0.01935
	F	0.31	0.00525	0.00525	0.00495
	G	0.10	0.00195	0.0018	0.0018
	H	0.03	0.0012	0.00105	0.0012

Yield (mg/mL): 4.362     Dilution Factor:  100×

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Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	2.13	1.859	1.702
	B	31.0	2.044	1.974	1.91
	C	33.0	1.438	1.428
	D	38.0	0.9098	1.061	0.9905
	E	43.0	0.01051	0.01111	0.0102
	F	46.0		0.0015	0.0012
	G	48.0		0.0015	0.0012
	H	50.0	0.0015	0.0012	0.0015

Download these data

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-07-29 12:13:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.