D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91N

Expression Data

Yield (mg/mL): 1.505

Kinetic Assay Data

**slope of I91N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0717	0.07275	0.07305
B	25.00	0.0519	0.0717	0.05595
C	8.33	0.04005	0.0405	0.03975
D	2.78	0.01665	0.0165	0.01905
E	0.93	0.00645	0.00555	0.00585
F	0.31	0.0021	0.00195	0.0021
G	0.10	0.0012	0.00105	0.0012
H	0.03	0.0009	0.0009	0.0012

Yield (mg/mL): 1.505 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by jgonz1074 and last updated on 2023-04-18 09:59:28.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91N A_{420 nm}**
	T (°C)	1	2	3
A	30.0	3.51E-01	3.48E-01	3.52E-01
B	31.0	3.01E-01	3.45E-01	3.38E-01
C	33.0		2.94E-01	3.06E-01
D	38.0		7.98E-02	7.92E-02
E	43.0	1.80E-03	1.80E-03
F	46.0			0
G	48.0			0
H	50.0			0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by jgonz1074 and last updated on 2023-04-18 09:52:54.

Reference Data for WT

**slope of I91N A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.