D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91G

Expression Data

Yield (mg/mL): 0.4478

Kinetic Assay Data

**slope of I91G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1178	0.1036	0.1057
B	25.00	0.087	0.08295	0.0795
C	8.33	0.1053	0.09135	0.0738
D	2.78
E	0.93
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 0.4478 Dilution Factor: 10×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2814	0.2931	0.2946
B	25.00		0.2127	0.2292
C	8.33	0.101	0.1119	0.1399
D	2.78	0.0384	0.04516	0.06135
E	0.93	0.009752	0.01155
F	0.31	0.0033	0.00315
G	0.10	0.00105	0.00105
H	0.03	0.00075	0.0009	0.00105

Yield (mg/mL): 4.691 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:36:42.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of I91G A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.