D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91M

Expression Data

Yield (mg/mL): 0.8651

Kinetic Assay Data

**slope of I91M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0339	0.0327	0.03465
B	25.00	0.02655	0.02745	0.0306
C	8.33	0.0192	0.01905	0.01845
D	2.78	0.0081	0.00795	0.0084
E	0.93	0.0027	0.0033	0.0033
F	0.31	0.00135	0.00165	0.0012
G	0.10	0.00105	0.00105	0.0009
H	0.03	0.00075		0.0006

Yield (mg/mL): 0.8651 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by melaniehorta and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91M A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.2169	0.1914
B	31.0	0.2037		0.1794
C	33.0
D	38.0	0.1074	0.0621	0.0921
E	43.0		0.0033	0.0027
F	46.0		0.0012	0.0012
G	48.0	0.0015		0.0012
H	50.0	0	0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by melaniehorta and last updated on 2023-04-18 10:06:53.

Reference Data for WT

**slope of I91M A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.