D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91H

Expression Data

Yield (mg/mL): 3.692

Kinetic Assay Data

**slope of I91H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1751	0.1794	0.1553
B	25.00	0.132	0.1368	0.1471
C	8.33	0.081	0.08267	0.0873
D	2.78	0.03405	0.0357	0.039
E	0.93	0.01035	0.01185	0.01215
F	0.31	0.00345	0.0033	0.00345
G	0.10	0.00135	0.00195	0.00165
H	0.03	0.00105	0.00105

Yield (mg/mL): 3.692 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by aruiz262 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91H A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.8487	0.7972	0.8478
B	31.0	0.75	0.7681	0.7017
C	33.0	0.7521	0.7536	0.6546
D	38.0	0.1182	0.1158
E	43.0		0.0021	0.0021
F	46.0	0.0012		0.0015
G	48.0	0.0018	0.0018	0.0018
H	50.0		0.0015	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by aruiz262 and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of I91H A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.