Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 8.69

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3629	0.3841	0.3977
	B	25.00		0.2968	0.2928
	C	8.33	0.2353	0.2502	0.2245
	D	2.78	0.08985	0.09615	0.09105
	E	0.93	0.0312	0.02805	0.02925
	F	0.31	0.0066	0.0066	0.00765
	G	0.10	0.00255	0.0024	0.003
	H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69     Dilution Factor:  100×

Download these data

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	2.629	2.366	2.391
	B	31.0	2.386	2.284	1.911
	C	33.0		2.137	1.923
	D	38.0	0.7872	0.8292	0.669
	E	43.0	0.0075	0.0078	0.006903
	F	46.0	0.0012	0.0018	0.0015
	G	48.0	0.0015	0.0018	0.0018
	H	50.0	0.0015	0.0018	0.0012

Download these data

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.