Raw Data for BglB Variant I91Q

Expression Data

SDS-PAGE
 3.466

Kinetic Assay Data

slope of I91Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1801 0.1800 0.1871
B 25.00 0.1323 0.1381 0.1476
C 8.33 0.08295 0.08340 0.08895
D 2.78 0.03495 0.03510 0.03615
E 0.93 0.01110 0.01125 0.01140
F 0.31 0.003300 0.003750 0.003450
G 0.10 0.001350 0.001500 0.001350
H 0.03 0.001800 0.001200 0.001350
 3.466      100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by aorti228 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3629 0.3841 0.3977
B 25.00 0.2968 0.2928
C 8.33 0.2353 0.2502 0.2245
D 2.78 0.08985 0.09615 0.09105
E 0.93 0.0312 0.02805 0.02925
F 0.31 0.0066 0.0066 0.00765
G 0.10 0.00255 0.0024 0.003
H 0.03 0.0009 0.00135 0.00165
 8.69      100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of I91Q A420 nm
T (°C) 1 2 3
A 30.0 1.006 0.9057 0.9732
B 30.0 0.9186 0.9275 0.9876
C 31.0 0.7941 0.7977 0.8130
D 38.0 0.2589 0.2670 0.2589
E 43.0 0.003600 0.003900 0.003900
F 46.0 0.001200 0.001500
G 48.0 0.001200 0.001500 0.001500
H 50.0 0 0

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by aorti228 and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of I91Q A420 nm
T (°C) 1 2 3
A 30.0 2.629 2.366 2.391
B 31.0 2.386 2.284 1.911
C 33.0 2.137 1.923
D 38.0 0.7872 0.8292 0.669
E 43.0 0.0075 0.0078 0.006903
F 46.0 0.0012 0.0018 0.0015
G 48.0 0.0015 0.0018 0.0018
H 50.0 0.0015 0.0018 0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.


Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.