D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91Y

Expression Data

Yield (mg/mL): 2.714

Kinetic Assay Data

**slope of I91Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1743	0.1768	0.172
B	25.00	0.1398	0.1298	0.1482
C	8.33	0.09015	0.08865	0.08955
D	2.78	0.0357	0.0357	0.04635
E	0.93	0.01095	0.01125	0.01185
F	0.31		0.00345	0.00315
G	0.10	0.00135	0.00165	0.00135
H	0.03	0.00075	0.0009	0.0009

Yield (mg/mL): 2.714 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by CRmym2021 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91Y A_{420 nm}**
	T (°C)	1	2	3
A	30.0	1.001	0.9467	0.9132
B	31.0	0.8241	0.9509	0.9408
C	33.0	0.7401	0.8148	0.8727
D	38.0	0.2271	0.1884	0.201
E	43.0	0.003	0.003	0.0036
F	46.0		0	0
G	48.0		0.0036	0.0015
H	50.0		0	0

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by CRmym2021 and last updated on 2023-04-18 10:17:00.

Reference Data for WT

**slope of I91Y A_{420 nm}**
	T (°C)	1	2	3
A	30.0	1.587	1.559	1.571
B	31.0	1.354	1.332	1.491
C	33.0	1.729	1.428	1.373
D	38.0	0.5814	0.5826	0.5559
E	43.0		0.0057	0.0054
F	46.0	0.0015	0.0021	0.0024
G	48.0	0.0048		0.0021
H	50.0	0.0018	0.0021

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:37:51.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.