D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91A

Expression Data

Yield (mg/mL): 0.7342

Kinetic Assay Data

**slope of I91A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3675	0.4194	0.4156
B	25.00	0.2772	0.2688	0.2738
C	8.33	0.2582	0.3294	0.2688
D	2.78	0.00225	0.0024
E	0.93		0.00075	0.00075
F	0.31
G	0.10		0.0006	0.0006
H	0.03	0		0

Yield (mg/mL): 0.7342 Dilution Factor: 10×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by Giulia and last updated on 2023-04-18 10:35:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91A A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.2211	0.1974	0.2184
B	31.0	0.174	0.1941	0.1647
C	33.0	0.1563	0.1567	0.1338
D	38.0	0.0492	0.0306	0.0225
E	43.0	0.0018	0.0021	0.0015
F	46.0	0		0
G	48.0	0.0015	0.003	0
H	50.0	0.0021	0.0015	0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by Giulia and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of I91A A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.