D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91V

Expression Data

Yield (mg/mL): 1.879

Kinetic Assay Data

**slope of I91V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0897	0.1201	0.1206
B	25.00	0.0825	0.0945	0.08625
C	8.33	0.0603	0.06118	0.0567
D	2.78	0.0231	0.02505	0.0216
E	0.93	0.0072		0.00765
F	0.31	0.003	0.00255	0.0024
G	0.10	0.0012	0.00105	0.00105
H	0.03	0.00075	0.0009	0.0006002

Yield (mg/mL): 1.879 Dilution Factor: 10×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91V A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.6078		0.5877
B	31.0	0.5265	0.543	0.5031
C	33.0	0.5151	0.494	0.5007
D	38.0	0.2667	0.2694	0.2664
E	43.0	0.0036	0.0036	0.0039
F	46.0	0.0021	0.0018
G	48.0		0.0012	0.0015
H	50.0	0.0018	0.0018

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of I91V A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.629	2.366	2.391
B	31.0	2.386	2.284	1.911
C	33.0		2.137	1.923
D	38.0	0.7872	0.8292	0.669
E	43.0	0.0075	0.0078	0.006903
F	46.0	0.0012	0.0018	0.0015
G	48.0	0.0015	0.0018	0.0018
H	50.0	0.0015	0.0018	0.0012

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2023-09-11 10:21:43.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.