Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2814	0.2931	0.2946
	B	25.00		0.2127	0.2292
	C	8.33	0.101	0.1119	0.1399
	D	2.78	0.0384	0.04516	0.06135
	E	0.93	0.009752	0.01155
	F	0.31	0.0033	0.00315
	G	0.10	0.00105	0.00105
	H	0.03	0.00075	0.0009	0.00105

Yield (mg/mL): 4.691     Dilution Factor:  100×

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Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:36:42.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	1.587	1.559	1.571
	B	31.0	1.354	1.332	1.491
	C	33.0	1.729	1.428	1.373
	D	38.0	0.5814	0.5826	0.5559
	E	43.0		0.0057	0.0054
	F	46.0	0.0015	0.0021	0.0024
	G	48.0	0.0048		0.0021
	H	50.0	0.0018	0.0021

Download these data

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:37:51.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.