D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120S

Expression Data

Yield (mg/mL): 1.132

Kinetic Assay Data

**slope of W120S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0069	0.00735	0.0069
B	25.00	0.00345	0.00345	0.0036
C	8.33	0.0015		0.0015
D	2.78	0.0009	0.00075
E	0.93	0.0006	0.0009	0
F	0.31	0	0	0
G	0.10	0		0
H	0.03		0.00105	0.0009

Yield (mg/mL): 1.132 Dilution Factor: 10×

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by rmaga009 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.04455	0.04440	0.04365
B	25.00	0.03450	0.03405	0.03165
C	8.33	0.02565	0.02730	0.02445
D	2.78	0.009900	0.01230	0.01110
E	0.93	0.003600	0.003900	0.004200
F	0.31	0.002100	0.002400	0.001800
G	0.10		0.002100	0.001800
H	0.03		0	0

Yield (mg/mL): 1.196 Dilution Factor: 100×

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of W120S A_{420 nm}**
	T (°C)	1	2	3
A	30.0	3.17E-01	2.90E-01	2.59E-01
B	31.0	3.26E-01	2.82E-01	2.76E-01
C	33.0	2.79E-01	2.97E-01	2.96E-01
D	38.0	9.36E-02	1.04E-01	1.17E-01
E	43.0	1.80E-03	1.80E-03
F	46.0	1.50E-03	1.20E-03
G	48.0	9.00E-04	9.00E-04	9.00E-04
H	50.0		9.00E-04	9.00E-04

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2023-09-14 11:09:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Cristina Baldacci: <cbaldacc@fiu.edu>.