Raw Data for BglB Variant W120M

Expression Data

SDS-PAGE
 0.6564

Kinetic Assay Data

slope of W120M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.04245 0.0471 0.0453
B 25.00 0.0189 0.0213 0.02055
C 8.33 0.00825 0.0087 0.009
D 2.78 0.00315 0.0036 0.0036
E 0.93 0.00165 0.0015 0.00165
F 0.31 0.0006 0.0006
G 0.10
H 0.03 0.0006 0.0006
 0.6564      10×

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by raulreynagajr and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.04455 0.04440 0.04365
B 25.00 0.03450 0.03405 0.03165
C 8.33 0.02565 0.02730 0.02445
D 2.78 0.009900 0.01230 0.01110
E 0.93 0.003600 0.003900 0.004200
F 0.31 0.002100 0.002400 0.001800
G 0.10 0.002100 0.001800
H 0.03 0 0
 1.196      100×

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of W120M A420 nm
T (°C) 1 2 3
A 30.0 0.0057 0.0057 0.0054
B 31.0 0.0051 0.0051 0.0045
C 33.0 0.0033 0.0033 0.003
D 38.0 0.0018 0.0021 0.0015
E 43.0 0.0015 0.0012
F 46.0 0.0018 0.0015 0.0015
G 48.0 0.0015 0.0012 0.0018
H 50.0 0.0009 0.0012

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by raulreynagajr and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of W120M A420 nm
T (°C) 1 2 3
A 30.0 3.17E-01 2.90E-01 2.59E-01
B 31.0 3.26E-01 2.82E-01 2.76E-01
C 33.0 2.79E-01 2.97E-01 2.96E-01
D 38.0 9.36E-02 1.04E-01 1.17E-01
E 43.0 1.80E-03 1.80E-03
F 46.0 1.50E-03 1.20E-03
G 48.0 9.00E-04 9.00E-04 9.00E-04
H 50.0 9.00E-04 9.00E-04

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2023-09-14 11:09:52.


Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.