D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120L

Expression Data

Yield (mg/mL): 1.433

Kinetic Assay Data

**slope of W120L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0198	0.0168	0.0201
B	25.00	0.0075	0.0069	0.00765
C	8.33	0.00375	0.0039	0.0039
D	2.78	0.00165	0.00165	0.0018
E	0.93	0.0015	0.00135	0.00105
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 1.433 Dilution Factor: 100×

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by DJDemps and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.04455	0.04440	0.04365
B	25.00	0.03450	0.03405	0.03165
C	8.33	0.02565	0.02730	0.02445
D	2.78	0.009900	0.01230	0.01110
E	0.93	0.003600	0.003900	0.004200
F	0.31	0.002100	0.002400	0.001800
G	0.10		0.002100	0.001800
H	0.03		0	0

Yield (mg/mL): 1.196 Dilution Factor: 100×

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of W120L A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.0441	0.042	0.0444
B	31.0	0.0519	0.0615	0.051
C	33.0	0.0363	0.0399	0.0408
D	38.0	0.0099	0.0105	0.0096
E	43.0	0.0018	0.0015
F	46.0	0.0009	0.0015
G	48.0
H	50.0

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by DJDemps and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of W120L A_{420 nm}**
	T (°C)	1	2	3
A	30.0	3.17E-01	2.90E-01	2.59E-01
B	31.0	3.26E-01	2.82E-01	2.76E-01
C	33.0	2.79E-01	2.97E-01	2.96E-01
D	38.0	9.36E-02	1.04E-01	1.17E-01
E	43.0	1.80E-03	1.80E-03
F	46.0	1.50E-03	1.20E-03
G	48.0	9.00E-04	9.00E-04	9.00E-04
H	50.0		9.00E-04	9.00E-04

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2023-09-14 11:09:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Cristina Baldacci: <cbaldacc@fiu.edu>.