D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120N

Expression Data

Yield (mg/mL): 0.2878

Kinetic Assay Data

**slope of W120N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00165	0.00165	0.00165
B	25.00	0.00105	0.00075	0.0009
C	8.33	0.00075	0.0007502	0.0006002
D	2.78		0	0.0006
E	0.93	0	0
F	0.31	0	0
G	0.10	0.00075		0.00075
H	0.03	0	0

Yield (mg/mL): 0.2878 Dilution Factor: 10×

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by dholl043 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0813	0.0813	0.07905
B	25.00	0.0699	0.05835	0.06105
C	8.33	0.04305	0.03855	0.03825
D	2.78	0.0177	0.0168	0.01665
E	0.93	0.0057	0.00525	0.00525
F	0.31	0.00195		0.00225
G	0.10	0.00075	0.0009	0.00075
H	0.03	0		0

Yield (mg/mL): 1.54 Dilution Factor: 100×

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of W120N A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.3893	0.3942	0.39
B	31.0	0.3714	0.3924	0.4113
C	33.0	0.3834	0.3633	0.381
D	38.0	0.1467	0.1716	0.1656
E	43.0	0.0024	0.0021	0.0018
F	46.0	0.0012	0.0015	0.0015
G	48.0	0.0012	0.0009	0.0015
H	50.0	0.0012		0.0015

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2023-09-11 10:24:31.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Cristina Baldacci: <cbaldacc@fiu.edu>.