Raw Data for BglB Variant S17A

Expression Data

Yield (mg/mL): 3.958

Kinetic Assay Data

slope of S17A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2159	0.2174	0.2181
	B	25.00	0.1749	0.1668	0.1749
	C	8.33	0.1434	0.1292	0.1509
	D	2.78	0.06375	0.06495	0.06225
	E	0.93	0.02865	0.0222	0.0285
	F	0.31	0.01095	0.01155	0.01065
	G	0.10	0.00465	0.00525	0.0048
	H	0.03	0.00165	0.00135	0.00165

Yield (mg/mL): 3.958     Dilution Factor:  100×

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ediaz205 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of S17A A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.7116	0.7176
	B	31.0		0.7095	0.735
	C	33.0	0.8223	0.66	0.771
	D	38.0	0.8331	0.7899	0.7987
	E	43.0		0.039	0.0333
	F	46.0	0	0.0036	0.0018
	G	48.0	0.0015	0.0015	0.0012
	H	50.0	0.0012	0.0015	0.0012

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ediaz205 and last updated on 2022-06-27 07:10:33.

Reference Data for WT

slope of S17A A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.3798	0.3789	0.3675
	B	31.0	0.3912	0.3699	0.3489
	C	33.0	0.3927	0.3734	0.3687
	D	38.0	0.267	0.2802	0.2775
	E	43.0	0.0054	0.006	0.0048
	F	46.0	0.0012	0.0012	0.0012
	G	48.0	0	0
	H	50.0		0.0012	0.0012

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by CCBestard and last updated on 2022-07-29 12:07:13.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.