Raw Data for BglB Variant N220F

Expression Data

SDS-PAGE
 2.62

Kinetic Assay Data

slope of N220F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2188 0.2152 0.2356
B 25.00 0.1896 0.1973 0.2117
C 8.33 0.2054 0.2025 0.2111
D 2.78 0.1845 0.1814 0.1845
E 0.93 0.1411 0.15 0.1516
F 0.31 0.0685 0.07905 0.07905
G 0.10 0.02985 0.03295 0.03315
H 0.03 0.00265 0.00395 0.00525
 2.62      100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nramesh and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3394 0.3278 0.3284
B 25.00 0.2976 0.2995 0.3014
C 8.33 0.3544 0.2882 0.2696
D 2.78 0.2086 0.2075 0.2209
E 0.93 0.1125 0.1292 0.1217
F 0.31 0.05035 0.05100 0.04540
G 0.10 0.02075 0.02190 0.01860
H 0.03 0.003500 0.002800 0.002150
 2.24      100×

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N220F A420 nm
T (°C) 1 2 3
A 50.0 0.0010 0.0008 0.0004
B 48.3 0.0007 0.0011 0
C 45.7 0.0275 0.0151 0.0414
D 42.4 0.2428 0.2368
E 37.7 0.8306 0.6484 0.8116
F 33.6 1.0180 1.0380 1.0740
G 31.3 0.9469 1.0700 1.1150
H 30.0 1.1820 1.2850 1.2110

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nramesh and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of N220F A420 nm
T (°C) 1 2 3
A 50.0 0 0.0005000 0.0007000
B 48.3 0.0009000 0.001100 0.0009000
C 45.7 0.005300 0.006600
D 42.4 0.2606 0.3161 0.3037
E 37.7 0.8638 0.9226 1.137
F 33.6 1.653 1.533 1.388
G 31.3 1.134 1.256 1.339
H 30.0 1.440 1.890 1.644

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:10:33.


Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.