Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.24

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3394	0.3278	0.3284
	B	25.00	0.2976	0.2995	0.3014
	C	8.33	0.3544	0.2882	0.2696
	D	2.78	0.2086	0.2075	0.2209
	E	0.93	0.1125	0.1292	0.1217
	F	0.31	0.05035	0.05100	0.04540
	G	0.10	0.02075	0.02190	0.01860
	H	0.03	0.003500	0.002800	0.002150

Yield (mg/mL): 2.24     Dilution Factor:  100×

Download these data

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0	0.0005000	0.0007000
	B	48.3	0.0009000	0.001100	0.0009000
	C	45.7	0.005300		0.006600
	D	42.4	0.2606	0.3161	0.3037
	E	37.7	0.8638	0.9226	1.137
	F	33.6	1.653	1.533	1.388
	G	31.3	1.134	1.256	1.339
	H	30.0	1.440	1.890	1.644

Download these data

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.