Raw Data for BglB Variant N220F

Expression Data

Yield (mg/mL): 2.12

Kinetic Assay Data

slope of N220F A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2178	0.2164	0.208
	B	25.00	0.1958	0.2026	0.1982
	C	8.33	0.1989	0.2045	0.1887
	D	2.78	0.1679	0.1557	0.172
	E	0.93	0.1281	0.1272	0.1479
	F	0.31	0.0708	0.0869	0.08805
	G	0.10	0.03215	0.03135	0.0359
	H	0.03		0.0005	0.00055

Yield (mg/mL): 2.12     Dilution Factor:  100×

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Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by sydneysbecker and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of N220F A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.0007	0.001	0.0009
	B