D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V79I

Expression Data

Yield (mg/mL): 3.63

Kinetic Assay Data

**slope of V79I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5289	0.5466	0.5234
B	25.00	0.4353	0.4463	0.4232
C	8.33	0.3687	0.4463	0.4118
D	2.78	0.2547		0.2522
E	0.93	0.115		0.121
F	0.31	0.04305		0.0505
G	0.10	0.01615		0.0146
H	0.03	0		0.0002

Yield (mg/mL): 3.63 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by mkazuma and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3811	0.418	0.4703
B	25.00	0.4223	0.3995	0.3932
C	8.33	0.3207	0.2511	0.4261
D	2.78	0.156		0.1696
E	0.93	0.05135	0.07115	0.0936
F	0.31	0.0109	0.0249	0.0392
G	0.10	0.00295	0.01275	0.01795
H	0.03	0.00025	0.00015	0

Yield (mg/mL): 2.17 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by mkazuma and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of V79I A_{420 nm}**
	T (°C)	1	2	3
A	50.0		0.0009002	0.001
B	48.0	0.001	0.001	0.001
C	46.0	0.0031	0.0032
D	42.0	0.2196	0.1915	0.1643
E	38.0	1.48	1.523	1.584
F	34.0	1.882	1.836	1.884
G	31.0	2.087	2.081	2.01
H	30.0	1.919	1.941	1.974

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by mkazuma and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of V79I A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.0008	0.001	0.0007
B	48.0	0.001	0.0008	0.001
C	46.0	0.002	0.002	0.0022
D	42.0	0.1373	0.1456	0.1652
E	38.0	1.149	1.084	1.096
F	34.0	1.506	1.445	1.462
G	31.0	1.403	1.288	1.414
H	30.0	1.462	1.586	1.429

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by mkazuma and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.