Raw Data for BglB Variant N293V

Expression Data

Yield (mg/mL): 2.05

Kinetic Assay Data

slope of N293V A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0299	0.03715	0.03405
	B	25.00	0.02865	0.0321	0.03055
	C	8.33		0.02045	0.01905
	D	2.78	0.0075	0.0093	0.0073
	E	0.93	0.0024		0.00275
	F	0.31	0.00085		0.00095
	G	0.10		0.0006	0.00055
	H	0.03	0.0003	0.0003	0.00035

Yield (mm): 2.05     Dilution Factor:  10×

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Protein purified on 11/21/21 and assayed on 11/18/21.
Data uploaded by dagalarza and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of N293V A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0006	0.0007	0.0005
	B	48.3	0.0006	0.0006	0.0005
	C	45.7	0.0006	0	0.0008
	D	42.4	0.0023	0.0029	0.0026
	E	37.7	0.0259	0.0248	0.0249
	F	33.6	0.0322	0.0319	0.0313
	G	31.3	0.033	0.0341	0.032
	H	30.0	0.0359	0.0369	0.0372

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Protein purified on 11/4/21 and assayed on 11/19/21.
Data uploaded by dagalarza and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.